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Description:
High Performance Liquid Chromatography is an analytical technique for separating compounds in a sample matrix. Separation is achieved by dissolving the sample in a suitable solvent and injecting it into a stream of solvent called the mobile phase. The mobile phase (and injected sample) is passed through a stationary phase (HPLC column). The compounds exit the column at different times based on the affinity the compounds have for the mobile phase and the stationary phase. Upon exiting the HPLC column they are detected and a signal is obtained electronically. There are multiple modes of separation including reverse phase, normal phase, ion exchange, and size exclusion.
Response at the detector of the HPLC is proportional to the amount of analyte injected. Analysis of samples in combination with standards with known analyte concentrations, allows for the concentration of the compound of interest to be determined.
Deliverable information:
Sample treatment: Direct injection with UV detection.
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Description:
High Performance Liquid Chromatography is an analytical technique for separating compounds in a sample matrix. Separation is achieved by dissolving the sample in a suitable solvent and injecting it into a stream of solvent called the mobile phase. The mobile phase (and injected sample) is passed through a stationary phase (HPLC column). The compounds exit the column at different times based on the affinity the compounds have for the mobile phase and the stationary phase. Upon exiting the HPLC column they are detected and a signal is obtained electronically. There are multiple modes of separation including reverse phase, normal phase, ion exchange, and size exclusion.
Response at the detector of the HPLC is proportional to the amount of analyte injected. Analysis of samples in combination with standards with known analyte concentrations, allows for the concentration of the compound of interest to be determined.
Deliverable information:
Sample treatment: Direct injection with UV detection.