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Ubiquitin (Ub) is ubiquitous. This small, 76-residue protein is used to mark cellular proteins for degradation by the ubiquitin proteasome system (UPS). Two key enzyme families, Ub ligases and deubiquitylation enzymes (DUBs), are responsible for putting on and taking off these Ub units and controlling the overall protein population of the cell. As you might imagine, this dynamic process is important, and dysregulation can lead to an array of bad things. Thus, understanding the relations between DUB or Ub ligase activity and specific diseases would be helpful in designing inhibitors or other therapeutic interventions. Accordingly, a number of Ub-based probes for activity-based protein profiling (ABPP) have been developed, typically involving an affinity tag, such as hemagglutinin (HA) or biotin, on the N-terminus and an electrophilic warhead on the C-terminus, such as propargylamide (PA), vinyl methyl ester (VME), or dehydroalanine (Dha). However, many of these probes are limited to experiments with purified recombinant proteins or cell lysates due to poor cell penetrance. Probes really need to be able to enter cells and react with enzymes intracellularly in order to gain the most useful information for drug targeting and other efforts.
| CAT.# | PRODUCT | CAT.# | PRODUCT |
| U2101 | Met1-Diubiquitin | U1030 | Ub-Rho100 |
| U2201 | K6-Diubiquitin | U1040 | Ub-Prg |
| U2301 | K11-Diubiquitin | U1050 | Ub-Br2 |
| U2401 | K27-Diubiquitin | UD2201 | Dha-K6-Diubiquitin |
| U2501 | K29-Diubiquitin | UD2301 | Dha-K11-Diubiquitin |
| U2601 | K33-Diubiquitin | UD2401 | Dha-K27-Diubiquitin |
| U2701 | K48-Diubiquitin | UD2501 | Dha-K29-Diubiquitin |
| U2801 | K63-Diubiquitin | UD2601 | Dha-K33-Diubiquitin |
| U1010 | Ub-AMC | UD2701 | Dha-K48-Diubiquitin |
| U1020 | Ub-ACC | UD2801 | Dha-K63-Diubiquitin |
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Ubiquitin (Ub) is ubiquitous. This small, 76-residue protein is used to mark cellular proteins for degradation by the ubiquitin proteasome system (UPS). Two key enzyme families, Ub ligases and deubiquitylation enzymes (DUBs), are responsible for putting on and taking off these Ub units and controlling the overall protein population of the cell. As you might imagine, this dynamic process is important, and dysregulation can lead to an array of bad things. Thus, understanding the relations between DUB or Ub ligase activity and specific diseases would be helpful in designing inhibitors or other therapeutic interventions. Accordingly, a number of Ub-based probes for activity-based protein profiling (ABPP) have been developed, typically involving an affinity tag, such as hemagglutinin (HA) or biotin, on the N-terminus and an electrophilic warhead on the C-terminus, such as propargylamide (PA), vinyl methyl ester (VME), or dehydroalanine (Dha). However, many of these probes are limited to experiments with purified recombinant proteins or cell lysates due to poor cell penetrance. Probes really need to be able to enter cells and react with enzymes intracellularly in order to gain the most useful information for drug targeting and other efforts.
| CAT.# | PRODUCT | CAT.# | PRODUCT |
| U2101 | Met1-Diubiquitin | U1030 | Ub-Rho100 |
| U2201 | K6-Diubiquitin | U1040 | Ub-Prg |
| U2301 | K11-Diubiquitin | U1050 | Ub-Br2 |
| U2401 | K27-Diubiquitin | UD2201 | Dha-K6-Diubiquitin |
| U2501 | K29-Diubiquitin | UD2301 | Dha-K11-Diubiquitin |
| U2601 | K33-Diubiquitin | UD2401 | Dha-K27-Diubiquitin |
| U2701 | K48-Diubiquitin | UD2501 | Dha-K29-Diubiquitin |
| U2801 | K63-Diubiquitin | UD2601 | Dha-K33-Diubiquitin |
| U1010 | Ub-AMC | UD2701 | Dha-K48-Diubiquitin |
| U1020 | Ub-ACC | UD2801 | Dha-K63-Diubiquitin |